EXPLORING THE WORLD OF
LIGHT
AND ELECTRON MICROSCOPY:
A HIDDEN WORLD EXPOSED

Microscopy Activity

You are invited to view the images below and work through the calculations given.

Please consider e-mailing answers and questions using the e-mail addresses provided.
terri.brown@fernbank.edu

HAIR  IMAGES INSTRUCTIONS

1.  You will need a metric ruler for this exercise.
2.  Measure in millimeters the magnified width shown in images A, B, & C below.
3.  Record on your paper the magnified width in millimeters of images A, B, & C.  You may find it easier to make measurements by printing this page.
4.  Determine the correct formula 1. or 2. required to calculate answers to A, B, & C.
5.  Using the appropriate equation show work involved in determining answer.  Provide answers using two (.00) decimal places.
6.  Be prepared to share your work your friends.
 
 
 
 

PLM 
Polarized Light Microscopy
   TEM 
Tranmission Electron Microscopy 
SEM 
Scanning Electron Microscopy
 A.  Actual width     is 1um B. Magnification = 8,000x  C. Magnification=5,000x 
Width in mm = _____  Width in mm = _____ Width in mm = _____
Determine the magnification. Determine the actual width. Determine the actual width.
Show calculations here:  Show calculations here:  Show calculations here: 
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.
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Answer: _____________ Answer: _____________  Answer:_____________

        FORMULAS

1.  Actual width = Magnified width
                               Magnification

2.  Magnification = Magnified width
                                 Actual width

METRIC SYSTEM CONVERSIONS

1 meter (m) = 100 centimeters (cm)
1 meter (m) = 1,000 millimeters (mm)
1 meter (m) = 1,000,000 micrometers (um)